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MSN2 gene transcription, Msn2p protein levels and cell viability increase under several stress conditions in the genetically modified strain.
Such a complex process of corrosion layer formation could explain the cell viability increase at day 21 for Mg2Ag, Pure Mg and Mg10Gd observed in our experiments.
Silver NP can reduce cell viability, increase the number of apoptotic cells, enhance reactive oxygen species (ROS) production, and activate caspase-3 in rat primary neurons.
In contrast, the AgNPs induced marked adverse effects on cell viability and could induce changes in cell shape, decrease cell viability, increase lactate dehydrogenase release, and finally cause cell apoptosis and necrosis [3, 6, 50, 51].
Our studies are consistent with previous studies which show that AgNP exposure could induce the changes of cell shape, reduce cell viability, increase LDH release, and finally result in cell apoptosis and necrosis [48,51,53,109-111].
The T. crispa (F1, F2, and F5) treatments show significant dose-dependent cell viability increase P ≤ 0.05.
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The intensity of the purple color increased over time as cell viability increased.
The mean pollen viability decreased significantly and the variation frequency of pollen viability increased to 75% until R3.
When ascorbic acid, a primary antioxidant, was introduced into the culture suspension, the ROS content decreased and cell viability increased.
By increasing the time of incubation, the loss of viability increased after 3 to 4 h of incubation.
Furthermore, as the industry's viability increases, major companies will begin to enter and offer more competition.
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