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To assess a potential synergistic effect, we measured the cell viability for single and combined treatments and calculated the combination index (CI).
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A platinum-based covalent viability reagent for single cell mass cytometry.
The average difference between cell viability data for single agent mitoxantrone is 12.9% between the two methods, while the average difference is 15% for the mitoxantrone-TRAIL combinations.
Fienberg, H.G., Simonds, E.F., Fantl, W.J., Nolan, G.P. & Bodenmiller, B. A platinum-based covalent viability reagent for single-cell mass cytometry.
Cell viability was simulated for single and combination drug treatments.
The reason is to be seen in the fact that both B-homozygotes have equal average s values which stay below the pertaining average B1 B2-viability for each single A-genotype.
It all sets the stage for a combustible coming election year, in which questions about the viability of single payer will be posed to gubernatorial candidates, incumbent lawmakers and new entrants to politics galvanized by the healthcare debate.
But, politically, there's been a huge shift within the Democratic Party over the viability of single-payer options, with support for the idea almost becoming a litmus test for potential presidential aspirants in 2020.
The cell suspension cultures maintained a minimum 65% single cell viability for 6 months when the cell suspension were sub-cultured as described above.
The MTT assay is well established for investigations of cellular viability in single cell cultures [3] and tissue slices [4], [5].
For single cell deposition DAPI (Sigma) was added for viability and left in solution prior to sorting in order to remove DAPI+ non-viable cells.
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