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While the resulting calibration curves had relatively high correlation coefficients, their use in various experiments resulted in viability estimates ranging from below 0% to far above 100%.
In addition, our absolute sperm viability estimates are consistent with male sperm investment in singly mated females [14].
Pollen viability estimates and sporad counts were performed using 1% acetic acid carmine stain.
The relationship between pollen viability estimates and self-pollinated seed production in three populations derived from microspore culture, self-pollination and open-pollination of the same trigenomic hexaploid hybrid resulting from the cross (B. napus × B. carinata ) × B.
Qualitatively, selection results during the two time periods were nearly identical (data not shown), but to maximize our power to detect selection, and to simplify the presentation of results, we use viability estimates from the second census as our measure of fitness.
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The cell viability, estimated by the trypan blue exclusion, was greater than 95% before each experiment.
Cell viability estimated by neutral red assay shows a similar trend as MTT assay.
Aliquots of about 100 µl of freshly isolated protoscoleces (roughly 50,000 larval worms of >95% viability, estimated by eosin exclusion) were incubated in 1 ml of RPMI 1640 containing penicillin/streptomycin (Sigma) for 3 h at 37°C with gentle agitation.
However, cell viability estimated by fluorescence of fluoresceine diacetate was not affected by Se-induced GSH depletion in roots tips.
In vitro assays of potency and viability estimate a maximum difference of 25 30% between fresh and cryopreserved PfSPZ Challenge (Table 1).
Cell density was assessed using a Fuchs-Rosenthal haemocytometer (Brand, Wertheim, Germany) and cell viability estimated by the standard trypan blue exclusion test.
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