To monitor cell viability, cells equivalent to 10−5 A600 units from mid-log and stationary phase cultures were plated on YPD/agar, incubated at 30°C for 48 h, and colonies were counted to extrapolate viable cell count per A600 unit.
Cell viability measurements were equivalent whether based on annexin V/PI or by live cell counts (Supplementary Fig. 6b).
Phenytoin-loaded alkyd nanoemulsions generally resulted in higher mean cell viability compared with equivalent concentration of phenytoin solutions, suggesting that the nanoemulsions provided a controlled-release property that maintained the optimum phenytoin level for keratinocyte growth.
We observed that sustained PERK signaling was detrimental to cell viability whereas the equivalent duration of IRE1 signaling was not, suggesting that extended PERK activity contributes to the cell death that occurs with chronic ER stress.
While the main objective of that work was to demonstrate that radical approaches can be used for nuclear reprogramming of somatic cells, we also established that cell viability is not equivalent to nuclear viability, as previously demonstrated for freeze-dried sperm [4].
Solvent DMSO did not affect cell viability, since concentrations equivalent to those present in the highest doses of curcumin (dilution 1/600) did not induce cell death.
Moreover, the Live/Dead kit's viability level which is equivalent to the percentage of intact cells evidences good cytoplasm preservation (also seen in Figure 1).
Cell shear sensitivity profiles were compared by subjecting cell culture material harvested from both the 5-L STR and the 70-L SUB at equivalent viabilities of ∼75% to conditions of low and high shear (Fig. 2B D).
Studies in breast and prostate carcinoma model systems indicate that tumor cells from metastasis suppressed kai1 or kiss transfectants, as well as their and metastatically competent control transfectants, arrived in the distant organ at comparable frequencies and with equivalent viabilities.
Established cultures (12 days) of BMSCs exhibited close, even equivalent, cell viability at each point of time from 1 to 12 days, as determined by cellular viability assays, and the difference of OD at 490 nm between ASp and HDs (HD1 and HD2) at each day (1 to 12 days) was not also statistically significant (P > 0.05, Student's t test for independent samples).
