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A significant viability effect on the offspring of MA females would make our estimates of conservative.
Similarly the viability effect of Evi depletion was abolished in the presence of conditioned medium (Fig S7B of Supporting information).
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Adverse cell viability effects remain restricted to the affected area.
To identify vulnerabilities relevant to specific cancer subtypes, we conducted a large-scale RNAi screen in which viability effects of mRNA knockdown were assessed for 7,837 genes using an average of 20 shRNAs per gene in 398 cancer cell lines.
By performing single pair matings, viability effects should be minimized.
In order to compare loss of viability effects in different cell lines, we normalised cell viability data from each cell line to the median of all effects in that cell line, representing each SMARTpool effect as a Z score [8] where Z = 0 represented no effect on viability and Z scores less than −3 represented significant loss of viability effects.
The aforementioned deviations from Mendelian expectations (Table 1) may be the result of viability effects of the mutant chromosomes or the wild-type chromosomes.
This suggests that viability effects of the mutations may not be entirely responsible for the excess of wild-type non-recombinant chromosomes observed in the other experiments [15], [17].
While it is widely expected that such extreme deformities should decrease the viability of affected flies, this has not been our experience in breeding Hsp90 buffered abnormalities generally [1], and no viability effects specific to the dfe trait were detected here.
Additional PcG alleles tested for female viability effects.
Several candidates showed severe cell cycle alterations in primary MSCs that led to the observed growth and viability effects.
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