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Cell viability determination is an important process in life science.
Cellular viability determination demonstrated greater induction of cell death by apoptosis and necrosis by Eclipse extracts in DCs.
In its "viability determination" for the company, the task force said G.M. had been "far too slow" to adapt, and that a "substantially more aggressive restructuring plan" was required.
For viability, mixing known numbers of non-viable cells with highly viable cells allowed evaluation of the specificity, accuracy and linearity of the viability determination.
Our experimental results suggest that evaluation of cell membrane integrity by electrical measurements provides a simple, quantitative and instantaneous method for cell viability determination, which could be very useful in fundamental cell death study as well as cell-based biosensors for toxic-detection.
Cell viability determination was made using MTT assay which was conducted for 120 h.
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The Trypan blue dye exclusion assay was used in all the cell viability determinations carried out.
After 24 hr, FL at 10(4), 10 -6), and 10 -8) M killed significant numbers of Sertoli cells as revealed by cell viability determinations.
Cells were plated in 96-well plates and cultured with indicated concentration of compounds for 3 days, followed by cell number and viability determinations as measured by CellTiter-Glo Luminescent Cell Viability Assay (Promega, Madison, WI, USA).
It is possible, however, that these cell viability determinations do not take into account damaged cells whose cell membranes are still intact because these cells would exclude the trypan blue dye until such point as membrane damage has occurred.
Conidial germination tests were conducted as the viability determinations described earlier but parallel plates were incubated at 36.5°C as well as 28°C and conidia examined at 18, 24, 48, 72 and 96 hr.
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