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Additionally, viability data is also provided in Supplementary Fig. S5 for our initially tested inhibitors.
The equivalent volume of the diluent was tested and caused no significant effect on cell viability (data not shown).
The knock-down did not reduce cell viability (data not shown) or affect the cell cycle profile (Supplementary Fig. 1), thus enabling further functional characterization.
In contrast, the transfection using reagent A and the SEAP expression plasmid shows the highest alteration of expression levels and lowest cell viability (data not shown).
For both, chitosan and alginate polymers, and for the nanoparticles, comparable cell viability data close to 100%, were obtained.
We also constructed a fourth model in which we assumed a minimum seed lifetime consistent with the seed viability data.
It also provides myocardial viability data, prognostic information, and risk stratification before major cardiovascular and noncardiac surgery.
All cell viability data were converted into percentages, and data for cells on tissue culture plate (TCP) in normal conditions (without high glucose, group TCP) were set as at 100%.
All cell viability data are presented as mean ± S.E.M. (n = 3).
In each panel in Figure 4, the cell viability data are shown as an inset.
In order to validate the cell viability data obtained by CellTiter-Blue, we used a second assay, trypan blue exclusion.
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