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The same amount of C. albicans served as viability control whereas triton-lysed mast cells served as a reference for 100% cellular death.
As a viability control, the model was replicated in static conditions using a conventional 12 well plate, and placed in the same incubator at the same time.
(e) Representative flow cytometry charts of (Lin− CD34+CD38−) HSC UCBs transduced with NAP1L3 shRNA (NAP1L3 shRNA), or a control vector (SC shRNA), stained with near-IR viability control and Annexin V, to detect apoptotic cells, five days post transduction.
Table 3 Percentage of cell viability by MTT assay Sample Percentage of viability Control 100 ± 0 CaP 98 ± 3 CaP-AgNP-0.25 97 ± 2 CaP-AgNP-0.5 94 ± 4 CaP-AgNP-1 86 ± 7 Open image in new window Fig. 9 Growth of L929 fibroblast cells in the presence of fabricated scaffolds CaP (a), CaP-AgNP-0.25 (b), CaP-AgNP-0.5 (c), CaP-AgNP-1 (d) and a control plate (e).
The viability control test for the HBHA synthetic peptides was performed by exposing PBMC to the CFP-10 peptide mixture with asetonitrile added at concentrations of 15% and 30% (vs. only 2.5% in the final peptide solution).
The cells were incubated in the absence of any other additives (Viability Control), in the presence of 50 ng/mL Stx2 (Stx2 Inhibition Control), in the presence of the test substance (30, 3 and 0.3 µM) (Substance Toxicity Control) and in the combined presence of the above amounts of Stx2 and test substances (Test) in 5% CO2 atmosphere with 100% relative humidity at 37°C for 10 hours.
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High surface area, appropriate hydroxyapatite formation, specific antibacterial action, increased cell viability, controlled swelling and degrading rate are favorably achieved at 2 1 nanocomposite ratio.
Tachyzoites were used in our study and their viability controlled by trypan blue dye exclusion and only parasite preparations with >95% viable cells were used.
Rotenone treatment decreased cell viability of control cells (60.1±0.6% of control) (Fig. 1B).
The test was accompanied by a viability positive control (RPMI plus cells) and negative control (RPMI plus extract).
Percentage of killing due to dexamethasone was calculated as: 100 × ((viability of control cells−viability of treated cells)/ viability of control cells).
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