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Without specific stage and viability markers, it is impossible to attribute a more precise developmental stage and viability condition to individually selected IRBCs under confocal observation.
The cost function measures how closely the model's behavior matches the viability condition.
The algorithm we propose starts from the definition of a viability condition and of a cost function.
Essential positive and negative feedback loops: No loop can be removed or substituted by a higher activation or degradation rate without violating the viability condition (21).
Depending on the biological model considered, the viability condition may include stability of a specific steady state, bistability [ 50], oscillations whose period lies in a given interval [ 20, 24], the production of specific gene expression patterns [ 22], and many others.
Essential positive feedback loop: The model keeps fulfiling the viability condition (21) after removing the negative loop or substituting this loop with a higher degradation rate of R p (see Additional File 1).
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Figure 1 Block scheme concerning the viability conditions.
We finish the paper with a block scheme that shows relations among viability conditions presented in the paper.
The utility of any rocking systems, as part of a building structure, depends upon the following viability conditions: 1.
An apoptotic cell stock was also created which may be used as a cell stock to achieve required cell viability conditions.
The viability in condition G (i.e., 74%) was roughly the product of the viability in conditions D and F (88% × 87% = 76%), suggesting an additive, rather than a synergistic effect of this combination.
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