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The aim of our study was to determine the more suitable procedure, surgical resection or liposuction, for harvesting human adipose tissue-derived stem cells (hASC) with regard to viability, cell count and differentiation potential.
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TSB viability cell counts represented an estimation due to their 3 –dimensional structure.
The resulting cell suspension was called NW. Viability, cell counts and flow cytometry analyses were conducted on this suspension.
Viability cell counts and CFSE tracking in 72-h-old cell cultures indicated that group O PBMCs (CD8+ and CD4+ T cells) underwent fewer mitotic cycles and had shorter telomeres than group Y (p = 0.04).
From weeks 3 to 5, the non-adherent iPS-MΦ produced were harvested every week and counted using NC-3000 Viandlity and Cell Count Assays (Chemometec) according to manufacturer's instructions.
We therefore shifted to counting the number of dead and live cells using the viability and cell count assay on the Nucleocounter 3000.
An added benefit would be that cathepsin K activity per se as well as the viability and cell count of osteoclasts and osteoblasts would remain undisturbed.
To assess the effects of PM on A549 cell viability, direct cell counting was performed.
Cell viability and cell counting were done using the trypan blue exclusion.
Hematocrit, leukocrit, cell viability, and cell counts of treated fish were not significantly different from those of control fish.
Cell viability and cell counts were controlled for all conditions after 24 hours with CellTiter-Glo® assay (Promega).
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