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Viability and responsiveness of PBMC was confirmed by an increase in cytokine production in response to LPS or PMA/ionomycin (data not shown).
We thus decided to develop a model of endometrial microhistoculture, adapted from the one elaborated by Menu et al [21] for the placenta, in which the complex interactions between epithelial, stromal and immune cells were preserved and the viability and responsiveness of the cells are verified.
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We show that suppression of multiple transcripts can occur simultaneously using an approach that preserves morphology, viability and glucose responsiveness.
These include the architecture of protein complexes and pathways, identification of expression changes compatible with viability, and the varying responsiveness to genetic perturbation.
Unfortunately, too often, the concern for the institutionalization, continuation, or sustainability of health programs, especially for their financial viability, eclipses attention to their effectiveness and responsiveness to public health needs.
Miki et al. showed that coculture of the mouse, rat and pig islets with MNNK-1 cells that served as a feeder cell in the coculture maintained their viability and insulin secretion with glucose responsiveness.
Different methods for the assessment of bacterial viability have been tested, including cellular integrity, metabolic activities, building of the cellular material, and responsiveness [3].
In the last decades, authors have detected the viability of bacterial cells based on metabolic activity, such as detection of RNA transcripts, a positive energy status and responsiveness.
Personality and responsiveness.
Emotional neglect meant lack of love and responsiveness.
And our experiences of future proofing, agility and responsiveness are informing service design throughout the council.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com