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In this study, we have compared the influence of penetrating and nonpenetrating cryoprotectants on the viability and functionality of encapsulated mesenchymal stem cells genetically modified to secrete erythropoeitin.
Production of liver-specific enzymes such as CYP1A1 and CYP3A4 after 14 days in culture indicates the viability and functionality of the encapsulated HepG2 cells.
These results suggest that the combination of small molecules maintained the viability and functionality of the ICAs in hypoxia by up-regulating HIF1α expression and down regulating the Caspase 3 activity.
We report here for the first time on the integration of a bacterial biofilm as the sensing element of a whole-cell biosensor, as a means to stabilize and preserve reproducibility, viability and functionality of the bacterial sensor cells.
Although probiotics have been demonstrated to be effective in antagonizing foodborne pathogens, challenges exist in the characterization and elucidation of underlying molecular mechanisms of action and in the development of potential delivery strategies that could maintain the viability and functionality of the probiotic in the target organ.
In addition, we assessed the viability and functionality of the placenta after particle perfusion.
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Difference in MWCNT uptake may affect cell viability and functionality to different extent, and further investigations are needed to clarify this. Figure 2 TEM photographs show the cellular uptake of MWCNTs.
We demonstrate that this combined approach resulted in increased viability, vascularization and functionality of the cardiac patch.
The viability, growth, and functionality of the 2D-cultured cardiomyocytes were evaluated.
A recent study from Germann et al. [ 17] showed that cryopreservation media complemented with bovine serum albumin (BSA) and in particular a combination of BSA and hydroxyethyl starch (HES) led to high viability, recovery and functionality of PBMCs in the ELISPOT as compared to PBMCs frozen with 90%% fetal calf serum (FCS).
These technologies range from the exploitation of innate in vivo functions to target and repair damaged tissue, to removal and purification of hMSC for use in numerous other settings such as tissue engineering, cell therapies, etc. Essential to these developments is the ability to process and maintain these unique biologics without the loss of viability and functionality.
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