The full experiment (i.e., transfection, drug treatment, viability assay, and data normalization) was repeated, resulting in high reproducibility Pearson's correlation coefficients ~0.70-0.80.
GS collected and analyzed the T98G cell viability and other data, and conceived of critical ideas for the study.
Percentage of inhibition for cell viability and ΔBliss data were calculated and plotted as a dose range for both drugs.
Dosing of drugs and DMSO was based on previous studies [ 24] and confirmed not to alter cell metabolism, viability and function (data not shown).
In Helianthus, [ 26], reciprocal translocations were described by conjugating observations of abnormal pairing in meiosis, studies on pollen viability and mapping data.
Means and Standard error of the mean (S.E.M). of viability and cytokine data were obtained and compared using one-way ANOVA, followed by the Bonferroni post-test in Graph-Pad PRISM 5.0.
After 5 to 7 days, no difference was observed between transduced cells exposed to 2 or 10 μg/ml of puromycin and transduced cells grown in medium without puromycin with respect to cell viability and morphology (data not shown).
After identification of adequate concentrations viability and MINC data were obtained for each compound, and experiments were repeated at this test concentration with two further cell preparations (Figs. 5, 6).
Results suggest that definitional uncertainties, the availability and viability of data, and the design of the method introduce inaccuracy, uncertainty and bias into data interpretation.
Zero insulin supplementation or concentrations <0.09 μM also reduced cell viability (Supplementary Figure S1A and data not shown).
