Sentence examples for via site specific from inspiring English sources

In the last two decades specific genetic engineering of mammalian cells via site specific recombinases such as Cre and Flp has been explored.

Therefore, it is possible that VPaI-1, VPaI-4, VPaI-5 and VPaI-6 may excise from the V. parahaemolyticus genome similar to E. coli PAIs, which have similar features, and form circular intermediates via site specific recombination between the direct repeats [ 30].

Regulation of rAAV expression by miRNA was primarily via site-specific cleavage of the transgene mRNA, generating specific 5′ and 3′ mRNA fragments.

With inspiration provided by natural protein systems, several artificial protein assemblies have been constructed via site-specific mutations or metal coordination, which have important applications in catalysis, material and bio-supramolecular chemistry.

That an engine of hard work powers a city of relaxation is a characterization that might inspire locals to protest via site-specific art, or to create a new eco-friendly blend of coffee using beans from Ecuador and Madagascar, with proceeds going to finance clever counterprogramming.

Additionally, scaffold-mediated lentiviral gene delivery was used to facilitate spatial organization of simultaneous chondrogenic and osteogenic differentiation via site-specific transduction of a single mesenchymal stem cell (MSC) population to overexpress either chondrogenic, transforming growth factor-beta 3 (TGF-β3), or osteogenic, bone morphogenetic protein-2 (BMP-2), transgenes.

In this context, the regulation of genome expression by small RNAs appears to be an important mechanism in the control of plant development (morphogenesis and phase transition), through post-transcriptional regulation of mRNA abundance (by miRNAs and secondary siRNAs) and silencing of gene expression (by siRNAs) via site-specific DNA methylation (Jones-Rhoades et al. 2006; Arikit et al. 2013).

The cellular localization of Rac1 is critical for specifying such diverse functions, via site-specific activation/inactivation and a range of protein interactions.

An RPN4* strain was constructed by replacing the rpn4Δ::LEU2 allele with an RPN4*::TRP1 cassette via site-specific recombination (Figure 2C).

The destination plasmids described here all replicate episomally in E. coli and integrate into the phage L5 attachment site of mycobacterial chromosomes via site-specific recombination [36].

As important agents of bacterial evolution, integrons are genetic elements that aggregate mobile gene cassettes via site-specific recombination.