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Via site directed mutagenesis, an hemolytic negative isogenic Serratia strain was generated to point out the importance of hemolysin production.
Five isolates were chosen to examine relative differences in genome size using flow cytometry: VIA (site 2), VIB (site 3), SIS (site 6), CCAP1085_21 (site 10), and CCMP3264 (site 12).
Isolates of T. rotula collected from the Mediterranean Sea (CCMP1647, site 11), from the coast of Vancouver Island (VIA, site 2), and from the Seto Inland Sea (SIS, site 6) were made axenic to remove contaminating bacteria.
Local economies would be rebuilt through the grounding of business via "site here to sell here" rules, funded by money made to stay predominantly where it was generated.
The mutations were introduced via site directed mutagenesis of a smaller fragment and then re-subcloned into the targeting construct to avoid potential mutations generated by PCR.
To demonstrate that miR-130a is required to mediate translational repression via site A in the FOG-2 3' UTR, we took two approaches.
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This is the first study that successfully increased transaminase activity in Asd via site-directed mutagenesis.
Combinations of three point mutations were then created via site-directed mutagenesis.
In fact, many of the FPs were obtained via site-directed mutagenesis from just a few wild-type sequences.
Four hu3S193 variants (I253A, H310A, H435A and I253A/H310A) were generated via site-directed mutagenesis and radiolabelled with diagnostic isotopes iodine-125 or indium-111.
To identify the mutation of amino acids in E110A/E134A related to its activity at low temperature, single mutants E110A and E134A were obtained via site-directed mutagenesis.
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