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Once the serogroups were identified, the results of a second round of reactions that used primers and probes for the flagellar antigen l genes, 1,2; e,h; g,m; d; e,n,x; and z10, and the Vi gene were used to identify individual serovars.
Among the REM genes, only VRN1, a class VI gene, is well characterized.
(vi) Gene expression.
The majority of collagen VI gene mutations impair the secretion and deposition of collagen VI in skeletal muscle and/or fibroblast cultures [26 28].
In the ERF subfamily, a small number of genes from Group VIII, a single Group VI gene and no Group VII genes showed inducibility by drought stress.
In this paper we discuss our findings with particular reference to: (i) the presence of the atp8 gene; (ii) nucleotide composition; (iii) strand patterns; (iv) origin of replication; (v) supranumerary ORFs; (vi) gene arrangement.
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Sporadic/dominant and recessive mutations in the three collagen VI genes, COL6A1, COL6A2, and COL6A3, are reported to be causative.
Although most genes of the putative prophage 3 were absent in all 10 Cronobacter strains tested by CGH, the region corresponding to the phage Vi genes (ESA_03041 ESA_03048) was present in C. turicensis and partially present in C. sakazakii strains 20, 701 and 767 (Figure S1).
In cluster VI, genes exhibited a minor decrease after 3 h.
This phenomenon was also observed with type VI genes, consistent with a ligand-mediated release of an activating complex.
Diagnosis of collagen VI related myopathies is complex and a definite assignment of collagen VI genes requires molecular genetic testing currently available in only a few medical centers.
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