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Vascular density (VD; number of vascular structures per mm) and perfused vessel fraction (PF) were established.
Rather, vessel fraction (vessel area multiplied by vessel number per area) should be causally linked with density variation.
A homogeneous and smaller diameter vessel fraction was collected from the finer filters (60 µm mesh) than from the coarser filters (150 µm mesh).
One caveat to these experiments is that thinned myocardium was also present, which is known to specifically affect the intramyocardial vessel fraction.
Vessel fraction has variously shown either negative or no correlation with wood density (Preston et al. 2006; Jacobsen et al. 2007 a ; Mitchell et al. 2008; Martínez-Cabrera et al. 2009; Poorter et al. 2010; Zanne et al. 2010; Gleason et al. 2012).
Examination of the material collected from the coarser and finer filters (150 µm and 60 µm mesh respectively) shows that the 150 µm filters retain a less pure (and generally larger diameter) vessel fraction than the 60 µm filters; the latter generate a more homogeneous and higher TEER monolayer consistent with it being derived from relatively pure capillary endothelium.
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Previous gene profiling studies of brain vessels had several limitations [9], [10], [11], [47] including use of whole vessel fractions, which contain many different cell types, and the lack of elucidation of peripheral endothelial profiles for comparison.
We also found that the long-time tumour and vessel fractions were almost identical for the small domain with the three choices of boundary conditions, although tumour growth was fastest in the doubly-periodic case (see Figure 8).
First, because these studies used whole vessel fractions, which contain many different cell types, the transcriptomes of the individual cellular components, the endothelial cells and the pericytes, are not yet known.
Microvessels were washed off the meshes with the enzyme mixture, centrifuged for 5 min at 240 g at 4 °C to remove enzyme, then resuspended in MEM-H 10% FCS and centrifuged again; the resulting vessel fractions were kept separate as '150s' and '60s', the latter giving higher TEER.
The density of the non-vessel fraction and tissue fractions within the non-vessel fraction are indicated hereafter by the subscript 'NV', e.g. densityNV, fibre fractionNV.
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