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The reacting dye is changing its fluorescent characteristics while undergoing a fast chemical reaction with the vessel content and therefore shows the micromixing indirectly.
Then, by relying on the formulated model, the aim is to design a suitable state observer, so that an estimation of the temperature of the cooking vessel content can be made online.
The vessel content was cooled and then poured onto acidified water (200 ml).
The mixture was occasionally stirred using a magnetic stirrer to ensure proper mixture of the vessel content.
Immunostaining for the endothelial cell marker CD31 revealed no difference in the blood vessel content of VEGF-B-expressing lesions, compared to control lesions (Figure 3a b).
Treatment with SkQ1 increased the vessel content up to the normal value (Fig. 6).
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These pictures show that the vessel contents can be considered to consist of three or four gross circulation vortices depending respectively on whether the angled blade agitator is pumping downwards or upwards.
On the other hand, the use of water deluge systems or passive fire protection (PFP) materials decrease heat flow to the vessel contents and can reduce or eliminate the risk of a BLEVE from occurring.
Using a hypodermic needle thermocouple (HN-7-K-TEF, J-KEM Scientific, Saint Louis, MO, USA) pierced through the gasket, we have also compared the internal liquid temperature profile of the reaction vessel contents in the ELIXYS to the profile inside the same vessel immersed in a traditional preheated oil bath (Additional file 1: Figure S2).
Vessels content were mixed and kept at room temperature for 12 h, then the vessels were introduced in the microwave digestion system and digested using a four-step digestion program: (1) 5 min at 280 W, (2) 5 min at 700 W, (3) 10 min at 1,050 W, (4) 1 min at 0 W.
It appears that in the absence of the stromal cells, respective tumors progressed more slowly because of their fewer stroma and lymphatic vessels content [ 94, 95].
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