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After mounting, venae cavae were allowed to equilibrate in the vessel chamber for at least one hour before pressure loading.
Once exteriorized, the isolated segments of rats MLVs were transferred to an isolated vessel chamber (modified Living Systems Instrumentation single vessel chamber model CH/1) filled with prewarmed to 38 °C D-MEM/F12 solution (Invitrogen Corp., Grand Island, NY, USA), and isolated MLV segments were cannulated and tied onto two carefully matched glass micropipettes (Gashev et al., 2004).
The vessel chamber bath (Living Systems TC-09S, Living Systems, Miami, FL, USA) containing PSS + albumin was gradually warmed and maintained at 37°C for the duration of the experiment.
During all experiments, segments of MLVs were constantly superfused and perfused with prewarmed 38 °C D-MEM/F12, and the isolated vessel chamber was constantly warmed to maintain a temperature of 38 ± 0.1 °C in the D-MEM/F12 surrounding the vessel.
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Vessel chambers were placed on the stage of an inverted Nikon Eclipse TS 100 (Nikon Instruments, Irvine, CA) microscope fitted with a video camera connected to a data acquisition computer.
Whether one can get better, better than what dark goes on in vessel, in chamber, the blind ride down the nerve — I'd have to take night, flood it with day and more day.
A section of conduit artery (~500 μm diameter), from half way down the main artery running the length of the left lung lobe, was dissected out and mounted between two pins in a small vessel myograph chamber (Danish Myotechnology).
Studies have coupled controlling oxygen tensions within vessel bioassay chambers to begin to understand how oxygen specifically hypoxia regulate NO function, and this context has identified red cells—specifically hemoglobin within as critical modulators.
Infrared endoscopy allows for visualization of intimal surfaces of blood vessels, cardiac chambers, and valves through flowing blood.
Results from a previous paper indicated that it is possible to detect changes in neutron source distribution initiated by an inadvertent withdrawal of outer control rod with in-vessel fission chambers located azimuthally around the core.
Experimental studies carried out to evaluate cement degradation are often conducted by an HPHT chamber (vessel) to simulate reservoir conditions.
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