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We conclude that antigen-induced bronchoconstriction leads to an increase in tracheobronchial permeability to macromolecules, and this effect is likely to be mediated by an increased paracellular as well as transcellular vesicular movement of large polar solutes across the airway epithelial barrier.
CHC appears to serve as a negative regulator of vesicular movement.
Actin filaments, along with their associated proteins, are essential for cellular morphology, motility and migration, phagocytosis, vesicular movement, cytokinesis, and molecular transport between the plasma membrane and the nucleus [15].
Alternatively, the relationship between ept and Dome localization could indicate a direct requirement for ept in Dome endolysosomal trafficking in much the same way that ESCRT mutants block the vesicular movement and lysosomal turnover of the Notch receptor [4] [6], [47].
Depending on its expression level, MyoVb Tail mutant leads to the relative but significant decrease in the number of events detected by the SSD approach, more evidently in the TIRF microscopy modality consistent with the fact that Myosin Vb acts primarily as a molecular motor, driving vesicular movement.
Importantly, this mechanism would explain the phenotypic similarity between EspG and nocodazole treatment, as both restrict vesicular movement beyond their budding site (Cole et al., 1996).
Similar(53)
Whereas Ca2+ release from the large-volume Ca2+ stores, such as the endoplasmic reticulum (ER) and Golgi apparatus, are preferred for signal transduction, Ca2+ release from the small-volume individual vesicular stores that are dispersed throughout the cell, such as lysosomes, may be more useful in local regulation, such as membrane fusion and individualized vesicular movements.
The intensity changes in this video seem to be fluctuations instead of discrete vesicular movements.
Pdr10 and Pdr15 were also implicated in membrane lipid organization [ 8, 9], and Aus1 and Pdr11 were found to mediate non-vesicular movement of plasma membrane sterol to the endoplasmic reticulum in S. cerevisiae, facilitating exogenous sterol uptake [ 10].
Key mechanistic steps that generate the eventual read-out of the assays are the insertion of sterol into the PM (requiring the ABC transporters Aus1p and Pdr11p), non-vesicular movement from PM to ER, and esterification at the ER requiring the ACAT enzymes Are1p and Are2p (Raychaudhuri et al., 2006).
Calyculin A did not inhibit vesicular motion.
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