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Consistent with these activities, bud14Δ cells display fewer actin cables, which are aberrantly long, bent, and latrunculinA resistant, leading to defects in secretory vesicle movement.
Although restricted within boutons, the vesicle movement was substantially faster in nonbouton areas, consistent with the observation that a sizable vesicle pool continuously transits through the axons.
The mechanism of how AQP phosphorylation leads to the recognition of the flagged protein and subsequent vesicle movement and membrane fusion is still unknown.
The slower time constant closely matched the time constant for the decrease of clathrin intensity and likely represents vesicle movement away from the membrane.
slowed vesicle movement, enabling us to count the number of photobleaching steps in a number of vesicles (Figure 2 A and B).
Further work is necessary to investigate the role of Toca-1 in controlling N-WASP actin polymerization activity, in vivo, during vesicle movement.
Similar(14)
Both targeted fluorescent proteins and acidic dyes have been used, separately or combined, to monitor the dynamics of secretory vesicle movements and their fusion with the plasma membrane.
As a proof-of-principle we altered the influences of vesicle movements and environment on autophagy.
A similar effect was found after inhibition of vesicle movements using BDM.
Vesicle movements were recorded for 5 min with a 1 s time-lapse interval using MetaMorph software (Molecular Devices).
In other cellular systems myosin Vb, Rab11a, and Spire proteins cooperate to drive actin-based vesicle movements and dynamics (Schuh, 2011; Montaville et al., 2014 perhaps similar mechanisms exist for mitochondrial movements.
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