Citrus greening disease is caused by "Candidatus Liberibacter spp.," including "Candidatus Liberibacter asiaticus (Las)." For detecting this disease, we designed new primers from the Las 16S rDNA and used a very small DNA template for PCR.
In some cases, stochastic effects due to the very small number of available templates, can give inconsistent results; e.g. for one of the replicates of sample P2-08-08, the 92 bp amplicon did not amplify in the multiplex endpoint RT-PCR assay but showed a band for the 161 bp product.
AB-PCR was amplified with very small amount of genomic DNA as a template and two oligonucleotides specific to boxes A and B of the promoter of RNA polymerase III as primers as described elsewhere [ 37].
Template-switching allows for cDNA preparation from very small RNA amounts, where even protocols have been published for the analysis of single-cells [ 4- 6].
3) Also, the observed conformational differences among some of these maps (e.g. S-CPV and CPV) are very small to support the hypothesis that 'the enlarged capsid may facilitate dsRNA template movement'.
In contrast, only a very small number (as little as one) master, source, or founder genes from which the proposed RNA template is transcribed are indeed multiplying THEMSELVES.
When very small amounts of DNA are present in the sample, stochastic effects can be the cause that the template DNA of some of the alleles is not sufficiently present in the PCR reaction.
Access to open content, including food safety training documents, generic HACCP models and record keeping templates, regulatory compliance guidelines, etc. provides a valuable starting point for small and very small food manufacturers, vendors, and suppliers.
Both co-clustering and template assignment identify small differences between similar samples, and solve the problem of enumerating an absent or very small population, e.g., 0 5 cells in a negative control.
Adding template-switching oligonucleotides to reverse transcription reactions is the most commonly used approach when working with very small quantities of RNA even from single cells.
