As is evident from the figure, the induction of SBD-1 mRNA expression was very rapid after the cells had been treated for 2 h and the greatest induction was observed at 6 h, which suggests that the highest expression levels were around 6 h.
Most significantly, p94 exhibits very rapid autoproteolytic activity in all protein expression systems examined to date [ 25, 26].
Introduction of the 35S-GFP construct into soybean cotyledons resulted in relatively high levels of gene expression, followed by a very rapid decline.
If exposure of UUA-containing mRNA to ribosomes limited for bldA tRNA elicits ppGpp synthesis as our results suggest, this could reduce the efficiency of transcription of any genes adapted for expression in conditions favouring very rapid growth.
Interestingly, the co-transfected HeLa SS6 cells died before GFP expression was detected indicating very rapid induction of cell death, in agreement with published data for HeLa cells [ 23].
This compound also induced a very rapid and substantial rise in the level of expression of p21waf1.
Clearance of goserelin following subcutaneous administration of the rapid-release solution formulation is very rapid.
Thus, a single MAP kinase coordinates diverse responses to stress that are temporally, spatially and mechanistically distinct, thereby providing very rapid initial relief, which facilitates subsequent changes in gene expression that permit long-term adaptation to harsh environmental conditions.
Interestingly, in all cases the increase in E2F1 expression in response to TGF β was very rapid, suggesting that TGF β induces post-translational protein stabilization of E2F1.
Thus, the strikingly low IRIP gene expression observed in Bd3-1 could be related to the very rapid transition from vegetative growth form to flowering observed in this line [ 4].
