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The range across individuals is small except at very low alignment bitscores (Table S1).
As seen in Figure 4a, Bowtie1 can exhibit very low alignment rates for these samples.
In these tables, we report the very low alignment rate of Bowtie2, which is at most about 13%.
We show that even at a low SNR level of 0.5 and a typical range of tilt angles between -70° and +70° our method can still achieve a very low alignment error (Table 1).
Alignment coverage varies considerably across the genome (fig. 1 A ) and is highly correlated across the two populations (fig. 1 B ). Regions with very low alignment coverage in both populations likely represent either repetitive regions with little sequence that can be uniquely mapped or regions that are difficult to sequence with 454 technology.
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The large size of DLP and its high symmetry make it an ideal specimen for this study, yielding high-contrast particles with very low-alignment errors (Henderson et al., 2011) and almost a thousand averaged subunits per particle.
These results exhibit a very low response alignment rate compared to males.
Since sequence identity among different DnaQ-H domains is very low, their alignment was first generated based on structural information as assessed by a CE-MC server [ 43] followed by manual adjustment based on published literature.
Without the secondary etching, very low levels of alignment and randomly aligned CNTs were grown in the SRR patterns.
The nucleotide identity between our P element and those previously isolated from Drosophila, Scaptomyza, Lordiphosa, and Lucilia genera is very low, and the alignment was not very reliable.
For the fish assemblies, the optical mapping results show very low proportions of alignable sequence, and alignable sequence is predominantly in the level 3 category, reflecting local alignments.
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