However, GST-GmPHD5 could also recognize both H3K4me and H3K4me3 with very low affinity, a result that is uncommon in other PHD finger domain containing proteins such as ING protein and BPTF [ 15].
The Fur4 mutant allele lacking transport and internalization had a very low affinity for uracil, indicating that lack of recognition of the substrate may have been the cause of defective internalization.
However, previous studies demonstrated that ACV has a very low affinity for human gGT, suggesting that additional targets may exist contributing to the biological activity.
More recently, Zehn et al. have shown using altered peptide ligands that very low affinity ligand are able to induce the full differentiation program of naïve CD8+ T cells leading to the generation of effector and memory T cells [6].
We hypothesize that the very low affinity of the binding of synaptobrevin 2 to epsin, which explains why such an interaction had not been described so far, may nevertheless be physiologically relevant when compounded by other interaction of epsin with the bilayer, other coat components, and ubiquitinated cargo proteins.
Since CEM.NKR cells showed very low affinity to NK cells, it was speculated that it lost an unknown cell surface antigen(s) for NK cell recognition [33].
Using altered peptide ligands, they have shown that Ags with very low affinity for the TCR are able to induce the complete differentiation of naïve CD8+ T cells into effector and memory cells.
Therefore, the data indicate that AtCERK1 shows very low affinity for chitooctaose, while AtLYK5 shows an affinity very close to the well-characterized chitin binding protein WGA.
It is true that having P2X7R very low affinity for ATP (km in the hundred micromolar range), conditions for its activation rarely if ever occur in healthy tissues, but it is now clear that on the contrary, extracellular ATP may reach levels well above the P2X7R km.
Therefore it is possible that the var6 protein possesses a very low affinity CSA binding domain.
