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He had used a very low camera angle, at thirty degrees from the horizontal, while setting the tilt to the maximum in order to obtain a very high depth of field.
In this study, we sequenced regions of the 16S rRNA gene at very high depth for a small number of samples (four), resulting in a majority of the estimated GIT microbial diversity being captured.
With very high depth coverage, this score could be used as an indicator of the allele frequency difference between lines.
It uses either one marker (sequenced at very high depth)[ 20] or many (generally randomly amplified)[ 21] to analyze phylotypes and/or functional categories[ 22].
Using our Agilent SureSelect data we picked 10 regions with very high depth (Additional file10: Table S10) and examined them against the pseudo gene lists from Yale and UCSC (http://www.pseugenes.com).
Luo et al. [ 5] fingerprinted BAC clones which were then assembled into contigs, while Jia et al. [ 21] used a very high depth of short sequence reads to develop sequence contigs/scaffolds.
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The experimental results confirm that SPIF of commercial polymer sheets at room temperature has potential for the manufacture of complex parts with very high depths.
The quality of sandstone at the Vicksburg Jackson Formation is low because of very high depths and temperatures, which result in diagenesis and loss of permeability (Loucks et al. 1986).
Furthermore, ustacks collapsed over 6000 repetitive SbfI RAD loci in the stickleback genome into a smaller number of loci with very high depths of coverage, as indicated by the long right tail of the distribution that stretches far beyond the truncated display in the figure.
It includes identification of 1773 transcription factor (TF -encoding genes from very high-depTF -encodingration de novo transcript sequences of chickpea desi genesype ICC 4958 (Chickpea Transcriptome Database, CTDB release 1.0, http://www.nipgr.res.in/ctdb.html).
While the detection of mosaic point mutations has been used for the validation of suspected mosaicism at specific genomic positions in rare disease and cancer (5, 6), reliable detection of small-scale mosaicism genome wide requires very high-depth whole-genome sequencing, which is not currently economical for widespread clinical application.
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