Fly thoraces were fixed with 4% paraformaldehyde for 10 min at 4°C and then were cut in half by dissecting vertically along the bristles in the middle of the thorax.
Right atrial trabeculae (one or two per appendage) were dissected and suspended vertically between an isometric force transducer (MLT0202, ADInstruments, Sydney, Australia) and a stationary stainless clip in a 200 ml organ bath filled with daily prepared Tyrode's modified solution containing (mM) 120 NaCl, 3.5 KCl, 1.1 MgCl2, 1.8 NaH25.7, 2.07 NandO3, 2.0 CaCl2, and 5.5 glucose.
Briefly, the tibialis anterior was carefully dissected, the mass measured (weight) and vertically mounted in a temperature controlled chamber (30°C), where it was immersed in a standard Krebs-Ringer bicarbonate buffer solution (Sigma) and continuously gassed with a mixture of 95% O2 and 5% CO2.
Intact diaphragm strips were dissected from the left costal diaphragm and mounted vertically in water-jacketed organ baths (Radnoti, Monrovia, CA, USA) containing Krebs-Henseleit solution (25°C, curare 50 mg/L, pH 7.40, NaCl 135 mM, KCl 5 mM, dextrose 11.1 mM, CaCl2 2.5 mM, MgSO4 1 mM, NaHCO3 14.9 mM, NaHPO4 1 mM, insulin 50 units/L, 95%O2/5%% CO2).
LV anterior or posterior papillary muscle was dissected free, mounted between two spring clips, and placed vertically in a chamber containing Krebs–Henseleit solution at 28°C and oxygenated with a mixture of 95% O2 and 5% CO2 (pH 7.38).
Left ventricular anterior or posterior papillary muscle was dissected free, mounted between two spring clips, and placed vertically in a chamber containing Krebs-Henseleit solution at 28°C and oxygenated with a mixture of 95% O2 and 5% CO2 (pH 7.38).
It had an incredible set that was basically a house dissected - it was like a house had been cut in half vertically, so you could see into all the floors of the house and all the rooms.
Dissected plains are common.
Doctors dissected his brain.
