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Imaging of the membrane microarrays was performed on a Nikon Eclipse 300 vertical microscope using a 4 × objective.
Imaging was performed on a Nikon Eclipse 300 vertical microscope, and analysis and quantitation were performed using Adobe Photoshop.
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Additional steel rods were attached to the microscope assembly for further rigidity once a site of labeled neurons was located, and only vertical microscopic movements were necessary.
Images were captured with Leica TCS SPE and Zeiss LSM510 Vertical confocal microscopes.
After staining, the coverslips were mounted with Fluoromount-G (Southern-Biotech) and the images were acquired on a vertical Axioskop-2plus microscope (Zeiss) or a confocal microscope (LSM510, Zeiss) under the same conditions to compare intensities.
The myofibrils were also vertical on the microscope stage.
Each dot corresponds to one of the pairs of promoters and shows the ratio of the noise level of the highest noise promoter to that of the lower noise promoter as measured by FACS (horizontal axis) and by microscope (vertical axis).
The steady-state polarization (P) of a fluorophore is defined by the ratio of I H− I V to I H+ I V, in which I H and I V are the emission intensities horizontal and vertical to the microscope stage.
This type of microscope employs vertical illumination, in which the light source is inserted into the microscope tube below the eyepiece by means of a beam splitter.
In an atomic force microscope, the vertical position of the piezoelectric tube scanner and thus the cantilever deflection is controlled using a 2-degree-of-freedom control design.
Fiber depth was measured by recording the vertical displacement of the microscope nosepiece while focusing on the top and bottom surfaces of the fiber.
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