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Knockdown of both zebrafish CXCL12 versions was achieved using 0.4 pmol of oligonucleotides against cxcl12a (5' TTGAGATCCATGTTTGCAGTGTGAA-3' TTGAGATCCATGTTTGCAGTGTGAA-3andcxcl12bCGAGTG-3') Confocal fluorescence images were obtained using a Zeiss AxioImager.M1 microscope fitted with a CascadeII camera (Photometrics) and VS-Laser Control, all controlled by VisiView software (Visitron Systems).
Linguistic equivalence across the various language versions was achieved continuously during the validation process.
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A panel consisting of the authors and two translators proficient in English completed this translation task and the consensus versions were achieved.
This youthful version was achieved by digitally translating the actor's facial movements into data for a simulacrum that here looks like an animated death mask.
The directed evolution of KikGR towards a monomeric version was achieved after 15 cycles of mutagenesis.
From both translations a consensus version was achieved.
we produced different versions, and kept on testing until a reliable version was achieved.
In a formal meeting, the committee discussed discrepancies until consensus on a prefinal version was achieved.
The differences in versions were minimal and this consensus version was achieved with unanimity and without difficulty.
The first author (TSB) compared the versions and discussed with translators about the divergences that were found, and a first Portuguese version was achieved.
The level 2 testing process was repeated following these changes and perfect correlation between HealthTracker and the independently programmed version was achieved for all variables.
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Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com