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If the number and distribution of such stems are significantly different from those that can occur in the randomly shuffled version of the alignment, then the alignment and thus the sequences are declared as ncRNAs.
For performance reasons, Denoiser provides a banded version of the alignment algorithm.
A printer-friendly PDF version of the alignment can be produced.
All alignments were created with a downloaded version of the alignment tool ClustalW 1.83 [ 33], obtained from.
Second, we show that even the simplest version of the alignment problem within this framework is computationally hard.
Herein, we describe a semi-automatic version of the alignment program DIALIGN that can take pre-defined constraints into account.
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Thus, a total of eight different versions of the alignment programs were tested (Table 1).
To understand better the localization of these sequences on the mouse genome, we compared stand-alone versions of the alignment programs BLAT, SSAHA, and MegaBLAST.
When testing for significance of the observed patterns through permutations, 100 versions of the alignment were generated in which the locations were randomly reassigned to isolates.
An improved version of the tree alignment method with extension to global and local forest alignments is given in Höchsmann et al. (2003) and implemented in the program RNAforester.
The online version of the sequence alignment program MAFFT [ 58] was employed to align the amino acid sequences and four outgroup sequences (XerC and XerD from Escherichia coli and Thiobacillus denitrificans [ 17, 41]) using the following settings: FFT-NS-I, and the BLOSUM45 model.
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