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This engineered enzyme has the catalytic versatility and oxidative ability of a high-reduction potential versatile peroxidase, with enhanced temperature and pH tolerance similar to that of a highly stable plant peroxidase.
versatile peroxidase.
For example, manganese-independent peroxidase (versatile peroxidase) was not detected in T. versicolor by Gavril et al. ([2007]).
Enzyme activities for lignin peroxidase, versatile peroxidase and dye-decolorizing peroxidase were not detected under the culturing conditions employed.
The released H2O2 can activate the lignin peroxidases, manganese peroxidase and versatile peroxidase, which in turn are able to oxidize the lignin components in the plant cell wall.
The identified mutants with higher oxidative tolerance and catalytic efficiencies would be helpful for further improving the oxidative stability of versatile peroxidase.
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Peroxide tolerant versatile peroxidases are required for industrial applications.
This might be due to the involvement of other extracellular enzymes such as versatile peroxidases (Siddiqui et al. 2014).
Versatile peroxidases (VP), a recently described family of ligninolytic peroxidases, show a hybrid molecular architecture combining different oxidation sites connected to the heme cofactor.
This class includes the secretory fungal peroxidases and is characterized by a wide homogeneity; for example the manganese peroxidases (MnP), lignin peroxidases (LiP) and the versatile peroxidases (VP) all belong to this class (Lundell et al. 2010; Martíınez 2002).
The family of extracellular ligninolytic enzymes typically includes lignin peroxidases (LiP, EC 1.11.1.14), laccases (EC 1.10.3.2), manganese peroxidases (MnP, EC 1.11.1.13), versatile peroxidases (VP, EC 1.11.1.16) and other accessory enzymes.
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