Consequently, FMCA has become a versatile tool for mutation detection [10], [11].
However both models make the point that interacting individuals characterized by a phenotype determined with a complex map (e.g., RNA folding) may evolve to lower degrees of mutational robustness at high mutation rates, as these solutions are more versatile.
We describe a highly efficient, fast, and versatile method to generate targeted mutations and perform precise genome engineering in less than 2 months.
This study also highlights the ability of these resourceful and versatile parasites to compensate for deleterious mutations through an intriguing reliance on molecular plasticity, which might also apply in other key events of the life cycle in Apicomplexa.
Enzyme mismatch cleavage is a versatile approach that is widely used in mutation and polymorphism detection, because it enables the detection of any allelic variations independent of the corresponding phenotype and restriction site.
The presented approach is very versatile for generating in-frame deletions, point mutations or insertions within bacterial chromosomes.
Versatile technologies exist for generating, identifying and characterizing mutations in the Drosophila retina, making this an unrivaled system for deciphering gene function.
In addition, the approach is also highly versatile, allowing introduction of one or several point mutations, insertions and deletions into even difficult targets, using a common set of materials and procedures.
nos-cas9 TH_attP2, CFD2, and TH_attP40 are highly effective for germline mutagenesis of both the nonessential gene e and the essential gene wls, suggesting that they are the most versatile transgenic Cas9 sources for creating heritable indel mutations with transgenic gRNAs.
The relatively high rate of incorrectly mapped 35 bp and 50 bp reads with 9% mutations detected for SSAHA2, renders CLC the most versatile and accurate program in our comparison.
The idea of genes accumulating new mutations in a clock-like manner provides a versatile tool for dating genetic events [1], [2] even when the clock is stochastic and its rate variable [3].
