Therefore, we initially verified uptake of BPA by glioma cells in vitro.
Having verified uptake, we attempted to inhibit bpa-miR-5364 bpa-miR-5364 bpa-miR-5364he mammalian host at 4–5 days p.i. Larvae were maintained in cusinge for 48 hours in the presence of an antisense RNA oL3gonucleotide (ASO) designed to inhibit bpa-mirecovered a sequence-scrambled control oligo and levels ofrom1_27305, Bm1_05425 and Bm1_25620 mRNA assessed by qRtheCR.
Previous work in our lab has verified cell uptake and distribution of the nanoprobes in clinically relevant mouse and human cell lines.
Cells were stained with crystal violet to ascertain that cell-abundant areas are clearly located above the magnet and Prussian blue staining verified iron uptake of VFFs in this experiment (Fig. 5b).
Three mice were euthanized one day post low dose aerosol to verify bacterial uptake of 50 to 100 CFU per mouse.
One day after low dose aerosol infection, three mice were euthanized to verify bacterial uptake of 50 to 100 CFU per mouse.
Audit data on EHC coverage was obtained from PCTs to assess the proportion of clients eligible for screening and to verify the uptake rate.
Further experiments to determine and verify the uptake rates used would be beneficial for further refinement of the model and in particular to aid simulations of perturbed states where uptakes can have a large impact on model behaviour.
In order to verify PI uptake and confirm cell viability, upon treatment, the cells were stained with the membrane permeable neutral vital dye Calcein-AM (CAM, Invitrogen), which is rapidly converted by cell esterases into calcein that fluorescences at 530 nm within a viable cell [ 47].
Although we could not directly verify these uptakes in M. tuberculosis, experiments have shown that a M. tuberculosis strain that lacks the ability to synthesize three amino acids (valine, isoleucine, and leucine) could persist in mice for four weeks [ 61].
In addition, we verified that the uptake of radioactive signal by WT peritoneal macrophages have progressively augmented, whereas the uptake by Gal-3−/− macrophages was permanently lower (Fig. 8).
