However, we recommend to check the hybridization-likelihood by verifying the sequences of both AONs using freely available software tools such as RNAstructure [ 48].
where the conditions on are similar to and He provided a valid method to verify the sequence of the variational functional, for the above problem is bounded in (see also [9, 10]).
To verify the sequence of the RT-PCR products, additional PCR experiments were performed with Pfu polymerase rather than Taq.
Ensembl also examines the resequencing reads that align to known SNP loci in order to verify the sequence of the resequenced individual at these locations.
To verify the sequence of the expressed mRNA for these paralogs, RNA from single copepods was isolated using the TRI reagent RNA isolation procedure (Sigma Chemical, Saint Louis, MO).
Electrocompetent E. coli TSA were transformed and plated on YEG-Cl agar containing kanamycin, and four colonies were picked to verify the sequence of their homology arms.
Heterozygous sequences that contained ambiguous aligned regions or multiple heterozygous nucleotide sites were identified and then cloned using PGEM T-easy vector system II (Promega) to verify the sequence of each sequence in heterozygous individuals.
In order to verify the sequence of the viral genome assembled from the Illumina sequencing data, six sets of overlapping primer pairs were designed to amplify the viral fragments.
To verify the sequence of the five selected genes putatively involved in flower development (VvTFL1, VvLFY, VvAP1, Vv AP3, VvPI) we cloned and sequenced the corresponding open reading frame of wild grapevine, as well as V. v. vinifera (Table 2).
Nuclear fragments that contained overlapping peaks (double nucleotide calls), were cloned using a pGEM-T Easy Vector System I (Promega) to verify the sequence of each haplotype in heterozygous individuals.
