A hydroponics split-root system was designed to verify the alterations in the expression of proteins in SQR-21-inoculated and un-inoculated watermelon roots.
We also conducted Western Blot analysis to verify the alterations of genes at the level of translation for selected genes with varying expression profiles.
To verify the alterations of gene expression at the mRNA level, which appeared on the microarray, we chose representative genes (Table 1) with varying expression profiles for real-time RT-PCR analysis.
To verify the alterations of gene expression at the mRNA level, which appeared on the microarray, we chose four genes (HIF1α, TNFα, MMP9, THBS2) with varying expression profiles for real-time RT-PCR analysis.
To verify the alterations of gene expression at the mRNA level, which appeared on the microarray, we chose representative genes with varying expression profiles for real-time RT-PCR and Western Blot analysis.
To verify the alterations of gene expression at the mRNA level, which appeared on the microarray, we chose representative genes (Table 1 and Table 2) with varying expression profiles for real-time RT-PCR analysis.
All constructs were sequenced to verify the alteration.
qRT-PCR and western blot analysis verified the alteration of RSK4 expression in target cells.
To investigate the effect of periostin, we verified the alteration of OA-related markers in cultured chondrocytes by stimulation with periostin [ 19].
To verify the alteration of the coefficient, the authors analyzed PFMR and PFFMR against age change, as shown in Figure 2. The age spectrum effected significant differences in all of the FM and FFM variables except the PFFMR.
To verify the microsatellite alterations, the experiments were done as duplicates and each PCR was repeated at least twice.
