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We agree that verifying the activity of the reagents prior to their use in our experiments is an important step.
The assay using purified reagents and verifying the activity of the recombinant factor V molecules was conducted under conditions where all factor Xa was saturated with factor Va, as described by assessing α-thrombin formation by the change in the absorbance of a chromogenic substrate at 405 nm (Spectrozyme-TH, 0.4 mM) (43, 45).
The assay verifying the activity of the recombinant molecules was conducted as described by measuring thrombin formation by the change in the absorbance of a chromogenic substrate at 405 nm (Spectrozyme-TH) monitored with a Thermomax microplate reader (Molecular Devices, Sunnyvale, CA) (16, 22).
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In this study, we experimentally verified the activity of twelve meiotically-active promoters out of fifteen candidate promoters, including ten new promoters.
To verify the activity of these compounds in living cells, we applied SINE compounds to HEK293 cells expressing a red fluorescent protein (mCherry) carrying a strong leucine-rich nuclear export signal recognized by XPO1, as well as a weak nuclear localization signal (NLS-mCherry-NES 61.
In addition to cataloguing these CEs, we characterized and verified the activity of three putative esterases including a CE1 (Fisuc_1771), a CE2 (Fisuc_1641), and a CE6 (Fisuc_2534).
To verify the activity of dynasore after 24 hour of incubation, the uptake of transferrin was measured in HeLa cells treated with a solution of dynasore incubated in growth medium for 24 hours.
In order to verify the activity of spores and investigate the early development of U. prolifera, the slides in the Petri dishes were observed at regular intervals to determine whether the spores were attached.
In addition to cataloguing these exo-hemicellulases, we characterized and verified the activity of two GH43 enzymes, including Fisuc_1769 as an arabinoxylanase and Fisuc_1994 as an arabinase (Table 3).
In order to verify the activity of PaCATB, we next measured the hydrogen peroxide decomposition in these transgenetic PaCatB strains.
It would be important to verify the activity of HPV by assessing viral oncogene expression and level of expression.
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