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As shown in Figure 7a, the NF-κB activity was induced by S100A8/A9 in a dose-dependent manner, verifying the activation of NF-κB by S100A8/A9.
BBR also induces PKD1 translocation from cytosol-to-plasma membrane, further verifying the activation of PKD1.
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To verify the activation of IGF signaling pathway by telomerase activity, we studied the activation of IGF downstream target proteins in hMSC-TERT versus primary hMSCs.
This variable, however, was not an object of verification, since the purpose of the study was to verify the activation of capital via relationship networks.
To verify the activation of both IRFs during primary EBV infection and upon restimulation, monocytes were stimulated once or twice with EBV and the presence of phosphorylated forms of IRF3 and IRF7 was evaluated by immunoblotting.
In order to verify the activation of the RTA-based toxins by HCV protease in-vivo, the model cell lines, induced or uninduced for NS3 expression, were treated with "PE-RTA-cleavage site-stalk peptide" or "PE-RTA- mutated cleavage site-stalk peptide".
We also verified the activation of caspase-3 and caspase-12 involved in ER stress signalling in preliminary experiments.
To further verify the activation of dFoxO by APP in vivo, we examined the expression of pro-apoptotic gene hid, a well-known transcriptional target of dFoxO.
To verify the activation of innate immune signaling by fusion protein rGP5-FljB, we conducted a TLR-5-specific bioactivity assay.
To corroborate these observations, we verified the activation of genes related to phenylpropanoid and flavonoids biosynthesis, which act as anti-pathogenic molecules [ 25].
To verify the activation of an ethylene response in the Ethrel-treated berries, the expression of the ethylene biosynthesis genes ACS1 and ACO1 as well as the expression of the ethylene receptor gene ETR2 was also analysed.
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