Sentence examples for verifying specificity from inspiring English sources

Immunostaining revealed LB2 expression in axons and GCs in vitro, which showed a dose-dependent reduction when lb2 mRNA translation was inhibited with a translation-blocking antisense morpholino (LB2MO), verifying specificity.

We analysed melting curves of reaction products to verify specificity of primer binding and thereby circumvent any issues of non-specific SYBR green fluorescence.

A melting curve was obtained to verify specificity of the PCR.

PCR products were analyzed by agarose gel electrophoresis to verify specificity of the products.

Isotype and secondary antibody controls were performed for each staining to verify specificity.

To verify specificity of pharmacological agents, we downregulated BK and IK1 channels in U251 cells using gene-specific siRNAs.

Melt curve analyses were performed for all SYBR Green PCR amplifications to verify specificity of the reaction.

Each sequence was designed to amplify a region nested within an amplicon produced from RT-PCR, and was screened using a BLAST inquiry to verify specificity.

Negative controls were included in which cells were incubated in the presence of secondary antibody only to verify specificity of staining.

To verify specificity, a parallel specimen was incubated under identical conditions, but in the presence of a 10-fold molar excess of the ALDH-inhibitor, diethylaminobenzaldehyde (DEAB).

Negative controls were included to verify specificity.