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When necessary, the PCR products were verified by cloning the fragments into a TA vector for verifying DNA sequencing.
As an internal control for verifying DNA quality isolated from the above cell lines, we performed PCR with primers specific for human β globin (HBG).
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The PCR samples were submitted to agarose gel electrophoresis to verify DNA amplification.
All plasmids were verified by DNA sequencing.
The recombinant plasmid pShuttle2-endostatin was verified by DNA sequencing.
All amplified sequences were verified by DNA sequencing (LGC Genomics, Berlin, Germany).
The construct was verified by DNA sequencing (GenScript, Nanjing, China).
Positive clones were verified by DNA sequence analysis.
The constructed plasmids were verified by DNA sequencing.
Clones were verified by DNA sequencing prior to expression studies.
The gene insertion was verified by DNA sequencing.
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