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To verify the patterns of late pollen-preferred expression in our candidate genes, we used two strategies: a promoter trap system and generation of transgenic plants under the control of selected promoters.
While phenotypic data support that CI is a significant determinant of adaptive outcome, genetic data are needed to verify the patterns and to inform evolutionary models.
However, we caution that the coverage amassed is far from complete, and efforts should be made to fill both taxonomic and geographical gaps in order to verify the patterns that we have identified.
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Further simulations aimed to verify the pattern of fluid flow (laminar versus turbulent flow).
In particular, the LDQ subscores can be useful to verify the pattern of dependence in patients with MOH which could be different between patients satisfactorily responding to the treatment and those who do not.
The finding also indicates that a large-scale study could be conducted to verify the pattern.
Since MALAT1 is a ncRNA that shows dimorphic expression in the TSP we felt it was an excellent candidate for a gene involved in TSD so we used quantitative real-time PCR (qRT-PCR) to verify the pattern of expression suggested by the macroarray.
We verified the patterns of expression by five conserved wheat miRNAs -- miR167a, miR397, miR156a, miR1852, and miR827a – plus three novel miRNAs -- Ta-miR021-2-5p, Ta-miR004-1-5p, and Ta-miR044-1-3p.
Non-parametric two-sided Mann-Whitney-U test was used to verify if the patterns of heteroplasmy were different between sample groups.
Additional studies are needed to verify whether the patterns we describe here are general or an artefact of our limited sample size.
But further experiments are also needed in people without ALS to verify that the patterns observed weren't specifically related to this disease.
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