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To investigate the reliability of cDNA-AFLP for detecting differentially expressed genes and verify the expression patterns observed in the cDNA-AFLP analyses, qRT-PCR analyses were carried out for 29 TDFs.
To verify the expression patterns of these predicted genes under drought stress, 8 differentially expressed genes were selected for qRT-PCR analysis and the primers used in this assay are listed in Additional file 17: Table S12.
RT PCR was also used to verify the expression patterns of eight putative orthologs to known Arabidopsis nectary-enriched genes.
qRT-PCR was performed to verify the expression patterns revealed by the RNA-seq study.
RT-qPCR was performed to verify the expression patterns revealed by the RNA-seq study.
RT-PCR was performed to further verify the expression patterns of the isolated peptidase genes.
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To verify the expression pattern of proteins observed in 2DGE, expression levels for the proteins TrkC, RACK1 and HSP90, were analyzed by Western blot analysis (Figure S4).
We further applied quantitative RT-PCR to cross-verify the expression patterns of 7 different miRNAs using 8 LCM selected epithelial cells.
We then applied quantitative RT-PCR to cross-verify the expression patterns of 7 different miRNAs using 8 LCM-selected epithelial cells.
Finally, we applied quantitative RT-PCR to cross-verify the expression patterns of 7 different miRNAs using 8 LCM-selected epithelial cells and found the excellent correlation of the fold changes between the two platforms (R = 0.996).
We verified the expression patterns of Sik1 in the SCN seen on the exon arrays using qPCR.
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