Sentence examples for verify sequence of from inspiring English sources

To verify sequence of products, we performed regular PCR and ran the PCR products on a 1.5% (w/v) agarose gel in parallel with 100 bp DNA markers before staining with ethidium bromide.

CRISPR-sgRNA design and donor plasmid cloning ∼10 d 1.1 Verify sequence of the planned targeting regions for the sgRNAs in the fly strain used and in the S2 cells by sequencing to identify potential polymorphisms compared with the published sequence.

Currently, the lower limit of pre-miRNA thermodynamic stability is widely used as a criterion for predicting and verifying sequences of RNA that constitute pre-miRNA [ 9- 12].

To verify the sequence of the RT-PCR products, additional PCR experiments were performed with Pfu polymerase rather than Taq.

Ensembl also examines the resequencing reads that align to known SNP loci in order to verify the sequence of the resequenced individual at these locations.

Electrocompetent E. coli TSA were transformed and plated on YEG-Cl agar containing kanamycin, and four colonies were picked to verify the sequence of their homology arms.

To verify the sequence of the expressed mRNA for these paralogs, RNA from single copepods was isolated using the TRI reagent RNA isolation procedure (Sigma Chemical, Saint Louis, MO).

DNA sequencing was performed to verify the sequences of the oligonucleotide inserts.

However, few studies have had the benefit of longitudinal data to verify the sequencing of effects between relationship quality and outcomes [ 47].

Therefore we verified that the sequence of all 3C products was correct (data not shown).

For determination of the conservation of the relevant site, we verified the nucleotide sequence of the binding site.