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However, population genetic studies are necessary to verify isolation.
The PVAC was also used to map the local PV and antrum potentials to verify isolation.
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DNA sequencing was utilized to verify the isolation of puc256G, the puc19 plasmid containing the 81116AI2- luxS ORF with the A256G nucleotide mutation.
To verify the isolation method efficiency, the acid fosfatase activity per mg of proteins was evaluated.
Even if the applications are verified in isolation, the system designer must verify the combined behaviour, leading to an explosion in design complexity.
Crosses to acd11/eds1-2 acd11/eds1-2d4-2 verified the isolandon of new eds1 acd11/pad4-2 acd11/pad4-2 as 10 other recessiverifiedwo dominantheuppressor locisolationated laz1-12).
Detection of endogenous Pdgfrα and Nes transcripts verified the isolation strategy.
Third, we verified electrical isolation of the PVs without the aid of a circular mapping catheter.
Lysosomal integrity was verified after isolation by measuring β-hexosaminidase activity latency, and only preparations with <10% broken lysosomes were used (Storrie & Madden, 1990).
The tti2 mutations were verified after isolation of genomic DNA, PCR with oligonucleotides 6061-1 and 5693-2, and sequencing of the PCR product using 6061-1 as primer.
Moreover, the correct piggyBac-mediated integrations at canonical TTAA target sites were verified by isolation of 5' and 3' insertion site sequences by inverse PCR (see Additional file 2).
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