Sentence examples for verify integration from inspiring English sources

To verify integration of tax in the host chromosome, tail DNA was digested with BamHI and EcoRI and analyzed by Southern blotting using the SK45 probe[61].

For PCR analysis, both nptII and gus genes were analyzed to verify integration of the transgenes.

BAC clones were digested with Sal I to verify integration of pLD53 vector (pLD53 introduces a SalI site).

PCR, using primers U1 and D1, was used to verify integration in the correct genomic location (when necessary, ORF-specific primers were also used).

Genomic DNA was extracted from strains Control-PPP-XYL, PGM2-PPP-XYL, Control-XYL and PGM2-XYL to verify integration at the correct loci using PCR.

The PCR product was transformed into the wild-type strain SN95 (Cand239), aNAT-resistantantransformantsts were PCR tested with oLC275/oLC2802 and oLC274/oLC2803 to verify integration of the cassette.

Southern analysis was used to verify the integration of the construct and determine the approximate copy number of the integrated cellulase genes.

Accordingly, H9 hESCs were transduced with Best1::GFP lentiviral reporter and ten individual colonies were chosen to verify the integration of Best1::GFP expression cassette by genomic DNA PCR (data not shown).

The primer pairs N3P-F (5′-CTGTCAACTTCAAGCTTGGG-3′) and GUS-R (5′-GCACGATACGCTGATCCTTC-3′) or N4P-F (5′-GCAGTGGTATTGTGACAGAC-3′) and GUS-R were used in the PCR reaction to verify the integration of the OsNCED3::GUS or OsNCED4::GUS constructs in N3P or N4P transgenic rice plants, respectively.

This procedure reviews the analysis of the radial and vertical build provided by the system code to verify critical integration interfaces, e.g. missing or too large gaps and/or insufficient thickness of components, and updates these dimensions based on results of more detailed analyses (e.g. neutronics, plasma scenario modelling, etc).

To verify the 3' integration fidelity, the primer 3'veF (5' –TACACAAACATACAAAAATAAAC-3'), specific to P. berghei DT 5'UTR, and the primer 3'veR (5' –ATAATACAACAAAATGGATA-3'), specific to a sequence downstream of the P. berghei MSP1 3'UTR targeting sequence, were used.