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To verify conditional regulation, CM mice were examined for MYC expression using quantitative real-time polymerase chain reaction (qRT-PCR).
A 2-fold reduction in the Wls transcript helped verify conditional deletion (Table 1).
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This extension can be used as a second step in an analysis to verify interaction, conditional on observing a significant MDR or MDR-PDT result.
After finding the minimum d-separate genes between start and end genes, we extend the statistical formula [ 14] to verify the conditional independency among genes.
Although it may be difficult to verify that conditional and unconditional P-value distributions coincide for real data, it may be useful to examine histograms of each (e.g. as shown in Fig. 3).
28 As explained in the appendix, in order to verify if conditional models were removing the fixed effects of each panel, 26 we fit models with different specifications, such as unconditional negative binomial regression models and conditional Poisson regressions with robust standard errors.
To verify that conditional tethering of MAD1 to kinetochores could delay anaphase onset like previously shown for direct fusion of MAD1 to MIS12 (Maldonado and Kapoor 2011), we added rapamycin to a population of cells in G2 phase and monitored mitotic progression by live cell differential interference contrast (DIC) imaging.
In contrast, Vglut2 expression in the cKO-Cre-GFP was represented almost exclusively by the knockout allele; in 28 out of 29 (97 %) Vglut2-expressing cells recombination had occurred leading to a deletion of Vglut2, thus verifying the conditional targeting of Vglut2 (representative examples shown in Fig. 4b).
This has been applied to Bacillus subtilis [ 4], with essential genes defined as those for which a mutant could not be obtained, in many cases being verified by conditional-lethal constructs.
To further verify that the conditional deletion was achieved, semiquantitative RT-PCR was performed using cDNA generated from P1 rib cartilage RNA (Figure S1C).
We will only find one child for each combination of covariates - not even twins will have the exact same measures - this is obviously not enough to verify the correct conditional coverage of a fitted PI.
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