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It should be emphasized that for this study we did not verify assignments by spiking natural samples with authentic commercial or synthetic standards, a common practice for final verification of compound identity by NMR.
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We have developed advanced graphical software for significantly accelerated and verified assignments of rotationally resolved rovibrational molecular spectra.
Manual inspection of MS/MS data was performed for proteins having only two peptide matches to verify sequence assignments.
It is noteworthy that, although CID data are utilized to verify glycopeptide assignments, in our method, we did not implement CID fragmentation rules in the scoring function, and only ETD data should be submitted to GPE for appropriate FDR analysis.
All of the MS/MS spectra of peptides that were assigned by MASCOT as potentially having been phosphorylated were examined manually to verify the assignment.
Both homonuclear and heteronuclear 2D NMR spectroscopy techniques have been used to assist and verify the assignment of minor organic compounds found in wine [25].
Baseline tests of reading skills were performed prior to the experiment to verify the assignment of subjects to the research groups.
Authentic AEP was added to the purified UPC to verify the assignment using P-31, C-13 and H-1 NMR.
For cellulose-degrading species annotated in IMG, we verified these assignments based on these publications.
Mass spectral analysis of C. clementina phloem proteins was employed to verify this assignment.
To verify random assignment, ANOVAs were conducted to compare participants' age and conceptual and problem-solving knowledge on the pre-test among the three conditions.
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