To verify alterations in the EGFR/HER-2 receptors and downstream signaling pathways, we analyzed protein levels of p-EGFR, EGFR, p-HER-2, HER-2, p-ERK1/2, ERK1/2, p-AKT, AKT, c-myc, and PCNA (Fig 5A).
We identified and verified alterations of the oropharyngeal microbiome and assessed their association with cirrhosis and pneumonia.
A hydroponics split-root system was designed to verify the alterations in the expression of proteins in SQR-21-inoculated and un-inoculated watermelon roots.
Thus, in order to exclude a false positive, we use the open field test to verify possible alterations in locomotor activity in rats caused by I. paraguariensis.
Integrating RNA and DNA genomic data allows us to verify genomic alterations in tumor samples and distinguish the genomic signatures of different tumor subtypes.
We also conducted Western Blot analysis to verify the alterations of genes at the level of translation for selected genes with varying expression profiles.
To verify the alterations of gene expression at the mRNA level, which appeared on the microarray, we chose representative genes (Table 1) with varying expression profiles for real-time RT-PCR analysis.
To verify if alterations in mRNA abundance were associated with AHR binding to regulatory motifs, a publicly available chromatin immunoprecipitation with DNA microarray (ChIP-chip) dataset (GSE11850) [ 50] was analyzed as described previously [ 42].
We obtained blood samples at three stages of the championship season, to verify whether alterations occur, and what the clinical relevance of these modifications could be, in particular in relation to their allergic status.
To verify the alterations of gene expression at the mRNA level, which appeared on the microarray, we chose four genes (HIF1α, TNFα, MMP9, THBS2) with varying expression profiles for real-time RT-PCR analysis.
To verify the alterations of gene expression at the mRNA level, which appeared on the microarray, we chose representative genes with varying expression profiles for real-time RT-PCR and Western Blot analysis.
