Initially exposing cells to vector resulted in transient expression that declined after 2 days, verifying the requirement of migration to sustain expression.
To verify the requirement for repetitive LTD in mediating the decreased numbers of synapses in cultured neurons, we compared synapse numbers at 14 DIV after single versus three times LTD induction (Fig. 4).
The abolishment of Tip-mediated reporter activation by the highly specific SFK inhibitor PP2 verified the requirement of Src-kinase activity.
To verify the requirement for the kinase activity of Akt (a downstream target of IGF1R) in the mitogenic action of estradiol, we used shRNA strategy and shRNA-resistant expression vectors.
We independently verified the requirement of ATM for cell migration and invasion using an xCelligence system that measures cell migration and invasion, as well as proliferation in real-time and in the same cell populations which provides standardized experimental conditions.
We propose an integrated approach for modeling and verifying the requirements of Self-Adaptive SysteModel Driven Engineeringgineering techniques.
Each of these three alternatives constructs an executable requirements model for verifying the requirements and results in higher-quality requirements.
Additional points at the edges of the DS were tested in order to verify the requirements for CQAs to be fulfilled.
This study aimed at verifying the requirements to construct a molecular representation that suits a detailed kinetic model, in terms of input data and model formulation.
To verify the requirements of LTA4H for substrates with unblocked α-amino group (a vital feature for aminopeptidases) we checked the ability of this enzyme to recognize substrates with secondary amine derivatives (Pro, Tic, Oic) and with modified main chain (Apns (2S,3S), β-Ala, 6-Ahx).
