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It is important to note that none of the hypotheses predicted in unexpected directions are nodes in the core Cell Cycle block, an observation that further verifies the cell proliferation literature model.
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To further verify the cell performance, EQE spectra of TiO2 ETL was obtained and presented in Fig. 4c.
Segmentation of OCT images and histology verified the cell type-specific degeneration process over time.
The recently introduced GafChromic® EBT radiochromic film model has been used to verify the cell irradiation dose deposition achieving a dose uncertainty of <5%.
To verify the cell apoptosis and oncosis rates induced by the Ats-loaded NPs, the percentages of early apoptotic (Q4), oncotic (Q2), necrotic (Q1), and live cells (Q3) were quantified by flow cytometry.
Intracellular Ca2+ concentration ([Ca2+]i) was monitored simultaneously to verify the cell commitment in the activation process.
Dual-color Flow cytometric analysis was used to verify the absence of smooth muscle cell contamination in our HUVEC cultures and in order to verify the cell surface expression of PDGFRβ after EPC-CM stimulation for 24 hours.
To verify the cell cycle progress was going normally after the serum deprivation, we had also detected the expression status of CyclinD1 protein (Figure 6B), a marker that reportedly can be detected in early G1 phase [18] [20].
Immunostaining of the sectioned oocytes with anti-integrin β1 antibody verified the cell surface expression of FLAG- integrin β1 protein.
Flow cytometric analyses were used to verify the cell membrane localization of ICAM-2 and ICAM-2 variants.
For each cell line, one coverslip was fixed at that time to verify the cell cycle arrest and lack of cyclin A expression (not shown).
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