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Sequence verified whole gene synthesis products of native and Gene Composer engineered versions of P38α and NS5B were used as PCR templates for cloning into vector pEU-E01.
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Some of the most down-regulated and up-regulated genes in CD90+ CP stromal cells compared to CD49a+ NP stromal cells and verified by whole tissue and CD26+ comparison are shown in Figure 2.
All staining patterns on FFPE sections were also verified in whole mount salivary gland tissues fixed in 4% paraformaldehyde and 5% sucrose in 1× PBS, subjected to immunocytochemistry, and imaged using laser scanning confocal microscopy (510 Meta, Zeiss or SP5, Leica) (Larsen et al., 2003; Daley et al., 2009; Daley et al., 2011; Daley et al., 2012; Sequeira et al., 2012).
High accumulation of the PMPC-b-PBMA/IR-780 nanoparticles in tumor tissue was verified by whole-animal NIR imaging.
Functional expression of BK and IK1 in U251 and U87 glioma cell lines and primary glioma cultures was verified using whole-cell electrophysiological recordings.
Nine days follow-up is further provided in additional file 2. The location and sizes of the respective tumors were verified by whole-body RFP fluorescence scans.
The positive transformant, which selected from the MD plate and verified by whole-cell PCR using primers ARG-1 and ARG-40 (Table 1), was used for the expression of human arginase I. First, Western blotting with anti-6 × His antibody was used to confirm that the main band of approximately 38 kDa was Arg I (Fig. 1d).
Together, the physical map and our analyses independently verify the whole genome shotgun sequence assemblies and provide a high degree of assurance to the published whole genome shotgun sequence project.
Third, after verifying the whole acknowledgement signals, the PT chooses a part of LNs, labels them as CNs, and sends ranging signal to them.
The goal of this paper is to provide a tool for verifying the whole EU GHGI and for simulating specific policy and forest management scenarios at EU level.
The two sub-models were compared with analytical models respectively, and filed test data was used to verify the whole model.
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